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cbd oil stomach cancer

2 School of Gerontology, Binzhou Medical University, Yantai 264003, China

Data curation, Z.P., M.L., X.L. and X.C.; Formal analysis, G.Q., L.Z. and M.X.; Writing—original draft, X.Z. and Y.Q.; Writing—review & editing, Q.Z. and D.L.

Xiaoyu Chen

To explore whether the observed inhibition of the proliferation effect of CBD is related to cell apoptosis, Hoechst 33258 staining of nuclei was performed. Fluorescence microscopy revealed that there were more abnormal nuclei in the CBD administration group compared to the control. These nuclei had distinct apoptotic features such as nuclear contraction, irregular condensation of chromatin, and apoptotic bodies ( Figure 4 a). We also analyzed the apoptotic rate of CBD-treated SGC-7901 cells using an Annexin V FITC/PI double staining kit and flow cytometry. We found that as the concentration of CBD increased, the percentage of apoptotic cells in the SGC-7901 population increased ( Figure 4 b,c). These results indicated that CBD effectively induced apoptosis in SGC-7901 cells.

Effect of CBD on reactive oxygen species (ROS) levels in SGC-7901 cells. (a) After 24 h of treatment with CBD, the changes in reactive oxygen species (ROS) content in SGC-7901 cells were determined using an ROS assay kit. (b) After staining with an ROS species assay kit, the fluorescence distribution in CBD-treated SGC-7901 cells was observed under a fluorescence microscope.

In the above experiments, we observed that CBD could effectively induce apoptosis in SGC-7901 cells. To explore the intrinsic mechanism of this effect, we performed western blot analysis to identify the core proteins associated with apoptosis. The results showed that CBD significantly reduced the expression of caspase-3 and caspase-9 at the same time and markedly increased the content of cleaved caspase-3 and cleaved caspase-9 ( Figure 5 a,b). We also investigated the proteins that activate the caspase family of proteins, such as cytochrome C and Apaf-1, by western blotting. The results showed that CBD remarkably increased cytoplasm cytochrome C and Apaf-1 protein expression levels in SGC-7901 cells ( Figure 5 c,d). These results implied that CBD induced SGC-7901 cell apoptosis through a mitochondrial-dependent apoptosis pathway.

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Yuan H., Ma Q., Ye L., Piao G. The traditional medicine and modern medicine from natural products. Molecules. 21, pii: E559 (2016).

The ER is an important organelle for protein synthesis and modification, lipid biosynthesis, and maintenance of intracellular Ca 2+ homeostasis 23 . When homeostasis is broken by changes in glycosylation, Ca 2+ depletion, oxidative stress, or accumulation of misfolded or unfolded proteins in the ER lumen, and ER stress is induced 24 . During accumulation of misfolded or unfolded proteins, the three receptors in the ER transmembrane, inositol requiring enzyme-1α (IRE1α), phospho-PKR-like ER-resistant kinase (PERK), and activating transcription factor 6 (ATF6), are removed from Bip to increase the expression of ER chaperones such as Bip and Glucose Regulated Protein 94 (GRP94), then, maintained their homeostasis by eliminating them 24,25 . Moreover, in the previous study, we found that expression of ER stress-related genes is increased in CBD-treated colorectal cancer cells 5 . Consistent with this, we showed that CBD increased ER stress-related genes in gastric cancer cells. CHOP, a key transcription factor for ER stress, regulates Smac 26 . Our results showed that CBD elevated CHOP levels and knockdown CHOP using siRNA partially increased CBD-induced XIAP reduction, indicating that CBD inhibits XIAP by stimulating ER stress.

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Co-immunoprecipitation

Kaiyon Bio Tech Co., Ltd, 226 Gamasan-Ro, Guro-gu, Seoul, 08308, Republic of Korea

Silke, J. & Meier, P. Inhibitor of apoptosis (IAP) proteins-modulators of cell death and inflammation. Cold Spring Harb. Perspect. Biol. 5, a008730 (2013).

Xu, Y. C. et al. Tissue microarray analysis of X-linked inhibitor of apoptosis (XIAP) expression in breast cancer patients. Med. Oncol. 31, 764 (2014).

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Kraemer, M., Broecker, S., Madea, B. & Hess, C. Decarbonylation: a metabolic pathway of cannabidiol in humans. Drug Test. Anal. 11, 957–967 (2019).

1974: a research study at the University of Virginia using cannabinoids from cannabis showed they can prevent the growth of malignant cancer cells.

Symptoms of Stomach Cancer
Stomach cancer is characterized by a growth of cancerous cells within the lining of the stomach. Stomach cancer occurs when normally healthy cells within the upper digestive system become cancerous and grow out of control, forming a tumor.

During a the study researchers used cancer cells that were resistant to chemotherapy, and dosed the cells with a synthetic form of delta-9 tetrahydrocannabinol (THC), one of the primary compounds found in cannabis.

(Information from www.cureyourowncancer.org Click here for full Article)

Symptoms of Advanced Stomach Cancer

Researchers noted a drastic reduction in the survival rate of the cells that were exposed to the synthetic THC. When conducting the study researchers found that larger doses of THC led to higher rates of cancer cell death.